General description
Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.[3]
Application
Transferrin human has been used:
• to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity[4]
• as a supplement in serum-containing/devoid medium for human thyroid cell culture[5]
• as a target glycoprotein to develop an in-gel hydrazinolysis method[6]
Biochem/physiol Actions
Transferrin mediates iron transportation from plasma to cells.[3] The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin.[3] The transferrin binds to specific cell surface receptors and are internalized by endocytosis.[1] Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded.[1][2] Transferrin is known to deliver iron to cells in a physiologically safe and effective form.
Other Notes
Partially iron-saturated
Preparation Note
60℃热处理10小时。
Analysis Note
通过琼脂糖电泳纯化。