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人肿瘤坏死因子-α,TNF-α human;Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

别名:Cachectin, Cytotoxin, Differentiation-inducing factor (DIF), Necrosin, Tumor Necrosis Factor

制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SRP3177
CAS号: 94948-59-1
MDL号: MFCD00148692
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¥4,522.75

产品描述

General description

TNF-α (tumor necrosis factor α) is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts.[1][7] It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2.[2] The gene is mapped to human chromosome 6p21.3.[10] Recombinant human TNF-α is a soluble 157 amino acid protein (17.4kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Application

TNF-α (tumor necrosis factor α) human has been used as a positive control for induction of proinflammatory genes in human intestinal and hepatic cell lines.[10]

Biochem/physiol Actions

TNF-α (tumor necrosis factor α) is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. However, it can be a tumor promoter as well as a tumor suppressor.[2][7] TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, apoptosis and diabetes.[3][4][5][6][8] It is also associated with mood and anxiety disorders.[9]

Sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 0.5mM DTT.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

性质

biological source  human
recombinant  expressed in E. coli
assay  ≥98% (HPLC)
 ≥98% (SDS-PAGE)
form  lyophilized
potency  ≤0.05 ng/mL ED50
mol wt  17.4 kDa
packaging  pkg of 50 μg
application(s)  cell culture | mammalian: suitable
impurities  <0.1 EU/μg endotoxin, tested
color  white
UniProt accession no.  P01375
shipped in  wet ice
storage temp.  −20°C
Gene Information  human ... TNF(7124)


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 3
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32
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