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乙酰胆碱酯酶来源于人 [AChE],Acetylcholinesterase human;recombinant, expressed in HEK 293 cells, lyophilized powder, ≥1,000 units/mg protein (Lowry)

别名:乙酰胆碱乙酰水解酶,乙酰基胆碱酯酶,AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl

CAS号 9000-81-1 Enzyme Commission (EC) Number 3.1.1.7 ( BRENDA | IUBMB ) EINECS 编号 232-559-3 MDL number MFCD00081268 NACRES NA.32
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): C1682
CAS号: 9000-81-1
MDL号: MFCD00081268
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¥8,205.25

产品描述

General description

Acetylcholinesterase (AChE) is a serine hydrolase,[6] which belongs to the carboxyl esterase family of enzymes.[1] AChE is localised at neuromuscular junctions and cholinergic brain synapses.[6]

Application

Acetylcholinesterase human has been used:
• as a standard protein to measure protein levels acetylcholinesterase AChE-R and AChE-S[3]
• to stimulate human fibroblasts[4]
• to study its in vitro catalytic activity and to determine the effects of metals, H2O2 and OH radicals on the activity[5]

包装

0.1 mg in serum bottle

Biochem/physiol Actions

Acetylcholinesterase (AChE) is regarded as a biomarker in neurotoxicity.[1] It is a modulator of nitric oxide signal transduction pathway and marker of membrane integrity and aging.[2] AChE, hydrolyzes choline esters. It terminates the impulse transmission at cholinergic synapses. AChE does this by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE inhibitors prevent the cholinesterase enzyme from breaking down ACh and increases the level and duration of the neurotransmitter action.[6]

乙酰胆碱的主要降解酶体内。将乙酰胆碱+氢2O转化为胆碱+乙酸。

Unit Definition

在37 °C条件下,当pH为8.0时,一个单位每分钟将1.0 μ摩尔的乙酰硫代胆碱水解为硫代胆碱和乙酸。

Physical form

本产品以冻干粉形式供应。自pH为8的50mM磷酸盐缓冲液中的0.22 μm过滤溶液冻干。

Analysis Note

使用乙酰胆碱作为底物获得的活性是用丁基胆碱获得的活性的 30-100 倍,使用来自电鳗的乙酰胆碱酯酶。

性质

Quality Level200
recombinantexpressed in HEK 293 cells
formlyophilized powder
specific activity≥1,000 units/mg protein (Lowry)
mol wt64.6 kDa
storage temp.−20°C
Gene Informationhuman ... ACHE(43)


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32
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