Acetylcholinesterase (AChE) is a serine hydrolase, which belongs to the carboxyl esterase family of enzymes. AChE is localised at neuromuscular junctions and cholinergic brain synapses.
Acetylcholinesterase human has been used:
• as a standard protein to measure protein levels acetylcholinesterase AChE-R and AChE-S
• to stimulate human fibroblasts
• to study its in vitro catalytic activity and to determine the effects of metals, H2O2 and OH radicals on the activity
0.1 mg in serum bottle
Acetylcholinesterase (AChE) is regarded as a biomarker in neurotoxicity. It is a modulator of nitric oxide signal transduction pathway and marker of membrane integrity and aging. AChE, hydrolyzes choline esters. It terminates the impulse transmission at cholinergic synapses. AChE does this by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE inhibitors prevent the cholinesterase enzyme from breaking down ACh and increases the level and duration of the neurotransmitter action.
在37 °C条件下，当pH为8.0时，一个单位每分钟将1.0 μ摩尔的乙酰硫代胆碱水解为硫代胆碱和乙酸。
使用乙酰胆碱作为底物获得的活性是用丁基胆碱获得的活性的 30-100 倍，使用来自电鳗的乙酰胆碱酯酶。