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乙酰胆碱酯酶来源于人类红细胞 [AChE],Acetylcholinesterase from human erythrocytes;buffered aqueous solution, ≥500 units/mg protein (BCA)

别名:乙酰胆碱乙酰水解酶,乙酰基胆碱酯酶,AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl

CAS号 9000-81-1 Enzyme Commission (EC) Number 3.1.1.7 ( BRENDA | IUBMB ) EINECS 编号 232-559-3 MDL number MFCD00081268 NACRES NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): C0663
CAS号: 9000-81-1
MDL号: MFCD00081268
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产品描述

General description

Acetylcholinesterase (AChE) belongs to the carboxyl esterase family of enzymes. The erythrocyte AChE is membrane bound.[2] AChE is mapped to human chromosome 7q22.1.[3] It is enriched in aged erythrocytes.[4]

主要以四聚体糖蛋白形式存在,由二硫键连接的同型二聚体组成,单体分子量约为 80 kDa。

Application

Acetylcholinesterase from human erythrocytes has been used in:
•  cholinesterase inhibition assay for screening 4-aminoquinoline based compounds[5]
•  AChE activity assays to test the effect of positive allosteric modulators (PAMs)[6]
• organophosphorus compounds based inhibition assay[7]

乙酰胆碱酯酶 (AChE) 已被用于磷酰胺酸酯作为 AChE 抑制剂的构效关系研究。[1]

Biochem/physiol Actions

Acetylcholinesterase (AChE) is regarded as a biomarker in neurotoxicity.[2] It is a modulator of nitric oxide signal transduction pathway and marker of membrane integrity and aging.[4] The levels of erythrocyte (RBC) AChE are affected on pesticide exposure and in hemolytic anemia. RBC AChE is a marker in Hirschsprung′s disease and inflammation.[4]

乙酰胆碱酯酶是体内乙酰胆碱的主要降解酶。它将乙酰胆碱和水转化为胆碱和乙酸。胆碱酯酶被天然氨基甲酸酯生物碱、毒扁豆碱或毒扁豆碱抑制。

血液中存在两种胆碱酯酶:红细胞相关酶,它是一种真正的胆碱酯酶或乙酰胆碱酯酶 [(AChE)-E.C. 3.1.1.7],血清相关酶,它是假胆碱酯酶或丁酰胆碱酯酶 \ [(BuChE)-EC 3.1.1.8]。
乙酰胆碱酯酶是一种胞外酶,通过 GPI 锚定在红细胞膜上。

Unit Definition

一个单元将在pH7.4、37℃下每分钟水解1.0 μmol 乙酰硫代胆碱碘化物。

Physical form

溶于含 0.1%TRITON®X-100 的 20 mM HEPES (pH 8.0) 中

Preparation Note

该酶是借助 TRITON X-100 与其 GPI 锚一起提取的两亲性形式,并通过亲和层析纯化。

Analysis Note

以乙酰胆碱为底物的酶活力是以电鳗乙酰胆碱酯酶活力的 30-100 倍。

性质

Quality Level200
formbuffered aqueous solution
specific activity≥500 units/mg protein (BCA)
mol wt~80 kDa
storage temp.2-8°C
Gene Informationhuman ... ACHE(43)


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 190 | 欧洲Eur. ≥ 66 | 東京Tokyo ≥ 82 | 香港与北京 ≥ 55
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