丁基琼脂糖凝胶4FF,Butyl Sepharose 4 Fast Flow

丁基琼脂糖凝胶4FF是一种成熟的标准脂肪族疏水相互作用色谱 (HIC) 树脂,用于捕获和中间纯化较大的蛋白质。17098001;17098010;17098005;17098004;17098002
货号(SKU): 17098002
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₹ 19,525.39

Butyl Sepharose 4 Fast Flow is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification of larger proteins.

基于 Sepharose Fast Flow 基质的标准脂肪族 HIC 树脂,通过不带电荷的、化学稳定的醚键衍生。
BioProcess 树脂支持工业应用,并在批准的工艺中建立良好
Butyl Sepharose 4 Fast Flow 是 Sepharose Fast Flow HIC 平台的一部分,该平台在近几十年来一直是 HIC 色谱的工业标准。 Butyl Sepharose 4 Fast Flow 基于交联的 4% 琼脂糖。脂肪族配体通过醚键固定在基础基质上。配体不含带电基团,使真正的疏水相互作用色谱成为可能,而不会干扰离子效应。

Butyl Sepharose 4 Fast Flow 有多种不同的散装尺寸和方便的预包装形式,便于放大和工艺开发。作为 BioProcess 树脂系列的一员,Butyl Sepharose 4 Fast Flow 可通过供应安全性和全面的技术和法规支持满足工业需求


  • Standard aliphatic HIC resins based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Optimized for the separation of larger proteins in capture and intermediate purification steps
  • BioProcess resin supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding.

Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Butyl Sepharose 4 Fast Flow is based on cross-linked 4% agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

Butyl Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Butyl Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support


ParameterButyl Sepharose 4 Fast Flow
Chromatography techniqueHydrophobic Interaction Chromatography 
BioProcess resinYes 
Particle size, d50V1~90 μm 
Matrixcross-linked agarose, 4%, spherical 
Ligand Concentration~40 µmol Butyl/mL resin 
pH stability, operational23–13 
pH stability, CIP32–14 
Pressure/flow characteristics4150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water) 
Storage4 to 30°C, 20% Ethanol 
Chemical stabilityStable in commonly used aqueous buffers - 1.0 M NaOH, 30% isopropanol, 70% ethanol, 6 M guanidine-hydrochloride, 30% Acetonitrile, 1mM HCl 
Autoclavability20 min at 121 °C, 5 cycles in H₂O 
Certificate of AnalysisYes 
Pack size200 mL 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
  • 4The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure/flow test performed on the base matrix.
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16