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辛基琼脂糖凝胶4FF,Octyl Sepharose 4 Fast Flow

辛基琼脂糖凝胶4FF是一种成熟的标准脂肪族疏水相互作用层析 (HIC) 树脂,用于捕获和中间纯化较大的蛋白质。17094604;17094603;17094602
货号(SKU): 17094602
增值税发票 √ 免费送货 √ 订货电话:010-68689484
₹ 97,425.88

Octyl Sepharose 4 Fast Flow is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification of larger proteins.

脂肪族 HIC 树脂基于 Sepharose Fast Flow 基质,通过不带电荷的化学稳定醚键衍生。
辛基衍生物为 Sepharose Fast Flow HIC 产品系列增加了不同且互补的选择性
优化用于在捕获和中间纯化步骤中分离较大的蛋白质
BioProcess 树脂支持工业应用,并在批准的工艺中建立良好
Octyl Sepharose 4 Fast Flow 是 Sepharose Fast Flow HIC 平台的一部分,该平台在近几十年来一直是 HIC 色谱的工业标准。该树脂基于交联的 4% 琼脂糖。脂肪族配体通过醚键固定在基础基质上。配体不含带电基团,使真正的疏水相互作用色谱成为可能,而不会干扰离子效应。 Octyl Sepharose 4 Fast Flow 有多种不同的散装尺寸和方便的预包装形式,便于放大和工艺开发。

作为 BioProcess 树脂系列的一员,Octyl Sepharose 4 Fast Flow 通过供应安全和全面的技术和法规支持满足工业需求

 

  • Aliphatic HIC resins based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • The octyl derivative adds a different and complementary selectivity to the Sepharose Fast Flow HIC product family
  • Optimized for the separation of larger proteins in capture and intermediate purification steps
  • BioProcess resin supported for industrial applications and well established in approved processes

Octyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. This resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects. Octyl Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.

As member of the BioProcess resins range, Octyl Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

 

ParameterOctyl Sepharose 4 Fast Flow
BioProcess resinYes 
LigandOctyl 
Particle size, d50V1~90 μm 
Matrixcross-linked agarose, 4%, spherical 
Particle Size45 µm-165 µm 
Ligand Concentration~5 µmol Octyl/mL resin 
pH stability, operational23–13 
pH stability, CIP32–14 
Pressure/flow characteristics4150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water). 
Storage4 to 30°C, 20% Ethanol 
Chemical stabilityStable in commonly used aqueous buffers - 1.0 M NaOH, 30% isopropanol, 70% ethanol, 6 M guanidine-hydrochloride, 30% Acetonitrile, 1mM HCl 
Certificate of AnalysisYes 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
  • 4The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure/flow test performed on the base matrix.
商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16