丁基琼脂糖凝胶 [高性能],Butyl Sepharose High Performance

高性能丁基琼脂糖凝胶是一种脂肪族疏水相互作用色谱 (HIC) 介质,专为优先考虑高分辨率时的中间和精制步骤纯化步骤而设计。17543201;17543203;17543202;17543260;17543204
货号(SKU): 17543203
增值税发票 √ 免费送货 √ 订货电话:010-68689484

Butyl Sepharose High Performance is an aliphatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority

用于中间纯化和抛光步骤的脂肪族 HIC 树脂
选择性类似于 Butyl Sepharose 4 Fast Flow,但由于较小的 34 µm 珠尺寸而具有更高的分辨率
Butyl Sepharose High Performance 是 Cytiva 系列 HIC 树脂的成员,用于蛋白质的中间纯化和精制。 Butyl Sepharose High Performance 基于高度交联的 34 µm 琼脂糖珠,通过不带电荷的化学稳定醚键用脂肪族丁基修饰。它是一种真正疏水的树脂,显示出最少的离子相互作用。 Butyl Sepharose High Performance 树脂特别适用于中间纯化和精制步骤,由于粒径小,可提供高分辨率。

Butyl Sepharose High Performance 有多种不同的散装尺寸和方便的预包装形式,便于放大和工艺开发。作为 BioProcess 树脂系列的成员,高性能丁基琼脂糖凝胶通过供应安全性和全面的技术和法规支持满足工业需求


  • Aliphatic HIC resin designed for intermediate purification and polishing steps
  • Selectivity similar to Butyl Sepharose 4 Fast Flow but higher resolution due to the smaller 34 µm bead size
  • Relatively high ligand density for high dynamic binding capacity even at relatively low salt concentrations
  • BioProcess resin supported for industrial applications
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding.

Butyl Sepharose High Performance is a member of the Cytiva range of HIC resins for intermediate purification and polishing of proteins. Butyl Sepharose High Performance is based on highly crosslinked, 34-µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages. It is a truly hydrophobic resin displaying a minimum of ionic interactions. Butyl Sepharose High Performance resins is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.

Butyl Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Butyl Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support


ParameterButyl Sepharose High Performance, 1 L
BioProcess resinYes 
Particle size, d50V1~34 µm 
Matrixcross-linked agarose 
Particle Size24 µm-44 µm 
Ligand densityApprox. 50 µmol butyl/ml gel 
pH stability, operational23–13 
pH stability, CIP32–14 
Pressure/Flow SpecificationBase matrix: 100-200 cm/h, 300 kPa, BioPilot 60/600 column, bed height 30 cm 
Storage4 to 30°C, 20% Ethanol 
Chemical stabilityStable in commonly used aqueous buffers - 1 M sodium hydroxide, 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% acetonitrile, 70% ethanol, 3 M ammoniumsulfate, 1 mM HCl, 30% isopropanol, 2% SDS 
Pack size1 L 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16