β-葡萄糖苷酶来源于杏仁，β-Glucosidase from almonds；lyophilized powder, 10-30 units/mg solid

General description

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β-Glucosidase from almonds belongs to the family 1 of the glycoside hydrolases. Almonds contain prunasin hydrolases (PH) and amygdalin hydrolase. Nine types of PHs are associated with almonds. PHs have signal peptide sequence and correspond to molecular weight in the range 50 to 75 kDa. PHs also have ITENG, NEP and INKKGIEYY motifs conserved and have N-glycosylation sites.

Application

β-Glucosidase from almonds has been used:
•  in enzyme inhibition studies by 1,5-dideoxy-1,5-imino-d-xylitol(DIX) derivates
• as a control in for comparison of soybean isofavone glycosides degradation by β-Glucosidase from Talaromyce leycettanus
• as a medium component during saccharification and fermentation of yeast

β-葡萄糖苷酶还可用于合成葡萄糖苷和岩藻糖苷（在制药、化妆品和洗涤剂行业具有多种潜在的应用）、从类黄酮和异黄酮糖苷中水解除去糖苷配基部分、增强果汁和葡萄酒的风味、以及寡糖的生物合成。

包装

100, 250, 1000 units in glass bottle

Biochem/physiol Actions

Prunasin hydrolase (PH) is a β-glucosidase that degrades prunasin to mandelonitrile and glucose. Amygdalin hydrolase hydrolyzes amygdalin to prunasin and glucose. β-Glucosidase is mesostable and is sensitive to temperature at 60 °C and above. It is highly sensitive to high pressure.

β-葡萄糖苷酶可参与β-D-糖苷中连接碳水化合物残基的β-糖苷键的水解。它们可将内切和外切葡聚糖酶产生的纤维二糖和纤维寡糖转化为葡萄糖。

Unit Definition

一个装置将在37°C、pH 5.0下从水杨苷中每分钟释放 1.0μmol葡萄糖。

Preparation Note

色谱纯化

性质

安全信息

符号

  GHS08

信号词 Danger

危害声明 H334

警示性声明 P261 - P342 + P311

个人防护装备 dust mask type N95 (US), Eyeshields, Faceshields, Gloves

危害码 (欧洲) Xn

风险声明 (欧洲) 42

安全声明 (欧洲) 22-24-36/37

RIDADR NONH for all modes of transport

WGK德国 WGK 1