β-烟酰胺腺嘌呤二核苷酸水合物 [氧化型辅酶I]，β-Nicotinamide adenine dinucleotide hydrate [β-NAD, β-DPN]；Grade I, free acid, 100% (dry weight)

General description

Nicotinamide adenine dinucleotide (NAD) is a ubiquitously^[5] found electron carrier^[4] and a cofactor. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.^[5]

Application

I级NAD（烟酰胺腺嘌呤二核苷酸）用于人类第IV类乙醇脱氢酶的动力学研究。^[3]

NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay.^[8] It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.^[9]

Biochem/physiol Actions

NAD is also involved in microbial catabolism.^[6] β-NAD acts as a substrate for various enzymes in several cellular processes.^[7]

The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo.^[1] It works as an electron acceptor.^[2]

电子受体

包装

根据固体质量包装。

Quality

纯度：以干重（酶促、CN-配合物和吸光度）计，100％ β-NAD；A₂₅₀nm - A₂₆₀nm：0.83 ± 0.02，A₂₈₀nm - A₂₆₀nm：0.22 ± 0.02
不含污染物：检测不到LDH抑制剂
有机溶剂： =0.05%（GC）

Specifications

吸光系数：在260 nm处为17.6 x 10³l x mol^-1 x cm^-1 ，333 nm处为5.9 x 10³l x mol^-1 x cm^-1（CN-配合物）

Preparation Note

储存条件（工作液）：在2至8℃下储存4周后，10 mg/ml的水溶液中至少含有99％的NAD

Other Notes

仅用于生命科学研究。不可用于诊断。

本品是普通型 NAD。

性质

安全信息