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β-半乳糖苷酶来源于大肠杆菌,β-Galactosidase from Escherichia coli [Lactase];lyophilized powder, ≥500 units/mg protein

别名:β-D-半乳糖苷半乳糖水解酶;乳糖酶;β-D-Galactoside galactohydrolase;Lactase

CAS号 9031-11-2 Enzyme Commission (EC) Number 3.2.1.23 ( BRENDA | IUBMB ) EINECS 编号 232-864-1 MDL number MFCD00130623 NACRES NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): G3153
CAS号: 9031-11-2
MDL号: MFCD00130623
增值税发票 √ 免费货运 [顺丰 联邦] √
¥4,142.99

产品描述

General description

β-galactosidase is a 465 kDa tetrameric protein.[2] It is coded by the LacZ gene.[4]

Application

β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI).[5] It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).[6]

β-半乳糖苷酶可用于衍生化,例如 IgG 的酶标记,无需事先透析或凝胶过滤。[1]

Biochem/physiol Actions

β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.[3]

β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。

包装

基于蛋白质含量的包装尺寸

Physical properties

四聚体的分子量为465 kDa(每个亚基116.3 kDa)

Unit Definition

一个单位将在 pH7.3,37°C 下每分钟将 1.0μ 摩尔邻硝基苯基 β-D-吡喃半乳糖苷水解成邻硝基苯酚和 D-半乳糖。

Physical form

用磷酸盐缓冲液和蔗糖稳定

性质

Quality Level200
recombinantexpressed in E. coli
formlyophilized powder
specific activity≥500 units/mg protein
mol wt465 kDa
storage temp.−20°C


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
RTECS LW5840000
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 88 | 欧洲Eur. ≥ 126 | 東京Tokyo ≥ 46 | 香港与北京 ≥ 62