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β-半乳糖苷酶来源于大肠杆菌,β-Galactosidase from Escherichia coli [Lactase];for enzyme immunoassay, lyophilized, powder, ~140 U/mg

别名:β-D-半乳糖苷半乳糖水解酶;乳糖酶;β-D-Galactoside galactohydrolase;Lactase

CAS号 9031-11-2 Enzyme Commission (EC) Number 3.2.1.23 ( BRENDA | IUBMB ) EINECS 编号 232-864-1 MDL number MFCD00130623 NACRES NA.54
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): 48275
CAS号: 9031-11-2
MDL号: MFCD00130623
增值税发票 √ 免费送货 √ 订货电话:010-68689484
$437.55

产品描述

Application

Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.

Biochem/physiol Actions

β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。

包装

Bottomless glass bottle. Contents are inside inserted fused cone.

Physical properties

四聚体的分子量为465 kDa(每个亚基116.3 kDa)

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C

Other Notes

Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590)[2]; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique[3]; Enzyme immunoassay of thyrptropin releasing hormone (TRH)[1]; Preparation of enzyme-antibody conjugates[4]

性质

Quality Level100
gradefor enzyme immunoassay
formpowder
qualitylyophilized
specific activity~140 U/mg
mol wtMr ~135000
containsphosphate buffer as stabilizer
sucrose as stabilizer
shipped inwet ice
storage temp.−20°C


安全信息

个人防护装备 EyeshieldsGlovestype N95 (US)
RIDADR NONH for all modes of transport
WGK德国 WGK 3
RTECS LW5840000
商品规格
属性名称属性值
储存温度 Storage temp.-20°C低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 33 | 欧洲Eur. ≥ 27 | 東京Tokyo ≥ 9 | 香港与北京 ≥ 16