α-酮戊二酸，α-Ketoglutaric acid [α-KG]；BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98.5%

Alpha-ketoglutarate (αKG), a vital intermediate in the tricarboxylic acid (TCA)/Krebs cycle, is located between succinyl-CoA and isocitrate.[1]

 

α-Ketoglutaric acid has been used:

• as a supplement in minimum essential medium α (α-MEM) to culture primary mesenchymal stromal/stem cells (MSCs) to study its effects on age-related osteoporosis[1]
• for chemical treatments to compare the treatment effects of several compounds on lifespan between manual and automated lifespan machine (ALM) platforms[2]
• as a printed chemotaxis ligand to screen chemotaxis receptors with high-throughput technologies and verify the interactions with surface plasmon resonance (SPR)[3]

Alpha-ketoglutarate (αKG), a nitrogen scavenger, is an important source of glutamate and glutamine.[4] It controls the synthesis of ATP and decreases the generation of equivalent (NAD+/NADH) in the tricarboxylic acid (TCA) cycle.[1] It promotes the synthesis of protein and prevents protein degradation in muscles. This immune nutrient factor participates in the general immune and collagen metabolism.[4] In aged rodents, αKG accelerates the healing of bone defects. It may possess potential anti-aging effects.[1] This Krebs cycle molecule can be used as a supplement in insect and mammalian cell cultures.