过滤
关闭导航

PDHK3, active, GST tagged human [PDK3];PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SRP5249
增值税发票 √ 技术支持 √ 质量保障 √ 电话:13910327035
¥5,909.71

说明

PDHK3或丙酮酸脱氢酶激酶3是PDHK家族的成员,其磷酸化和灭活丙酮酸脱氢酶(PDH)。PDH是一种线粒体多酶复合物,可催化丙酮酸的氧化脱羧,是负责调节哺乳动物碳水化合物燃料稳态的主要酶之一。HIF-1诱导PDHK3表达,导致线粒体呼吸的抑制。PDHK3在癌细胞中的强制表达导致乳酸积累和耐药性增加,而敲低PDHK3抑制缺氧诱导的细胞质糖酵解和细胞存活。

生化/生理作用

PDHK3 or Pyruvate Dehydrogenase Kinase 3 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. HIF-1 induces PDHK3 expression leading to inhibition of mitochondrial respiration. Forced expression of PDHK3 in cancer cells results in increased lactic acid accumulation and drugs resistance, whereas knocking down PDHK3 inhibits hypoxia-induced cytoplasmic glycolysis and cell survival.
 

外形

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
 

制备说明

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
 

法律信息

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
 
 

属性

重组

expressed in baculovirus infected Sf9 cells

产品线

PRECISIO® Kinase

测定

≥70% (SDS-PAGE)

形式

buffered aqueous glycerol solution

specific activity

10.2-13.8 nmol/min·mg

分子量

~69 kDa

NCBI登记号

运输

dry ice

储存温度

−70°C

Gene Information

human ... PDK3(5165)

 

安全信息

储存分类代码

10 - Combustible liquids

WGK

WGK 1

商品规格
属性名称属性值
储存温度 Storage temp.-70°C超低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32
商品标签 [tags]