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PDHK1, active, GST tagged human [PDK1];PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): SRP5247
增值税发票 √ 技术支持 √ 质量保障 √ 电话:13910327035
¥5,909.71

说明

PDHK1或丙酮酸脱氢酶激酶1是PDHK家族的成员,可磷酸化和灭活丙酮酸脱氢酶(PDH)。PDH是一种线粒体多酶复合物,可催化丙酮酸的氧化脱羧,是负责调节哺乳动物碳水化合物燃料稳态的主要酶之一。PDHK1是克隆β细胞中PDH的重要调节因子,PDHK1和PDH的活性对高效代谢偶联很重要。维持低PDHK1表达/活性并保持PDH处于去磷酸化和活性状态,对于β细胞达到最大葡萄糖刺激的胰岛素分泌所需的代谢通量速率非常重要。

生化/生理作用

PDHK1 or Pyruvate Dehydrogenase Kinase 1 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDHK1 is an important regulator of PDH in clonal beta-cells and the activity of PDHK1 and PDH are important for efficient metabolic coupling. Maintaining low PDHK1 expression/activity and keeping PDH in a dephosphorylated and active state, is important for beta-cells to achieve the metabolic flux rates necessary for maximal glucose-stimulated insulin secretion.
 

外形

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
 

制备说明

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
 

法律信息

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
 
 

属性

生物来源

human

重组

expressed in baculovirus infected Sf9 cells

产品线

PRECISIO® Kinase

测定

≥70% (SDS-PAGE)

形式

buffered aqueous glycerol solution

specific activity

1.4-2 nmol/min·mg

分子量

~70 kDa

NCBI登记号

application(s)

cell analysis

运输

dry ice

储存温度

−70°C

Gene Information

human ... PDK1(5163)

 

安全信息

储存分类代码

10 - Combustible liquids

WGK

WGK 1

商品规格
属性名称属性值
储存温度 Storage temp.-70°C超低温冷冻
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32
商品标签 [tags]