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蛋白G亲和琼脂糖凝胶,GammaBind G Sepharose

与 Sepharose 4B偶联的重组GammaBind G 2 型,用于实验室规模的抗体纯化。17088502;17088501;17-0885-02;17-0885-01
货号(SKU): 17088501
签订合同 √ 正规发票 √ 技术支持 √ 质量保障 √ 全程可追溯 √
¥10,650.00
  • Recombinant GammaBind G type 2 coupled to Sepharose 4B engineered for antibody purification at laboratory scale.
  • Binds to all IgG subclasses from human, mouse, and rat.
  • Binds total IgG from guinea pig, rabbit, goat, cow sheep, and horse.
  • Will not cross-react with other serum proteins such as IgM, IgE, IgA or transferrin.

与 Sepharose 4B 偶联的重组 GammaBind G 2 型,用于实验室规模的抗体纯化。
与人、小鼠和大鼠的所有 IgG 亚类结合。
结合豚鼠、兔、山羊、牛羊和马的总 IgG。
不会与其他血清蛋白如 IgM、IgE、IgA 或转铁蛋白发生交叉反应。

 

ParameterGammaBind G Sepharose
Chromatography techniqueAntibody affinity chromatography 
LigandRecombinant GammaBind G Type 2 Lacking Albumin-Binding Region 
Particle size, d50V1~90 µm 
Matrix4% agarose 
Particle Size45 µm-165 µm 
Ligand Coupling MethodCyanogen Bromide Activation 
Binding Capacity/ml Chromatography Medium:>18 mg human IgG/mL, 6 mg mouse IgG/mL 
Ligand density~3 mg GammaBind G, Type 2/ml drained Gel 
ApplicationBinds to all IgG subclasses from human, mouse and rat, Binds total IgG from guinea pig, goat, cow, sheep and horse, Include practically all of the current and projected applications of protein A sepharose, Analyze and purify classes, subclasses and fragments of immunoglobulins from Any biological fluid or cell culture medium and isolation of immune complexes 
pH stability, operational23–9 
pH stability, CIP32–9 
Storage2 to 8°C, PBS pH 7, 20% Ethanol 
Chemical stabilityStable to commonly used aqueous buffers and additives Such as 1 M Acetic Acid, 1% SDS and 6 M Guanidine Hydrochloride 
  • 1Median particle size of the cumulative volume distribution.
  • 2pH range where resin can be operated without significant change in function.
  • 3pH below 3 is sometimes required to elute strongly bound IgG species. However,  protein ligands may hydrolyze at very low pH.
商品规格
属性名称属性值
储存温度 Storage temp.2-8°C冷藏
全球实时库存 Availability √美国St. Louis ≥ 20 | 欧洲Eur. ≥ 16 | 東京Tokyo ≥ 5 | 香港与北京 ≥ 20