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氯化钙二水合物 [二水氯化钙],Calcium chloride dihydrate;BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

别名:细胞培养级氯化钙;二水合氯化钙;二水氯化钙;氯化钙水合物;水合氯化钙;干燥氯化钙

Empirical Formula (Hill Notation): CaCl2 · 2H2O CAS号: 10035-04-8 分子量: 147.01 EC 号: 233-140-8 MDL编号: MFCD00149613 PubChem化学物质编号: 24892994 NACRES: NA.71
制造商品牌: 西格玛 Sigma-Aldrich
货号(SKU): C7902
CAS号: 10035-04-8
MDL号: MFCD00149613
增值税发票 √ 技术支持 √ 质量保障 √ 电话:13910327035
¥430.82

说明

氯化钙(CaCl2)是生物化学中常用的试剂。它在许多生物学过程中发挥着重要作用,包括信号转导、肌肉收缩、维持细胞膜和细胞壁稳定性。CaCl2用于制备和转化有能力的大肠杆菌(E.coli),以及用质粒DNA或高分子量基因组DNA转染真核细胞。CaCl2介导的E。coli与质粒DNA pBR322进行了研究。CaCl2用于来自E。大肠杆菌。在CaCl2和柠檬酸钠存在下猪胰腺弹性蛋白酶的结晶揭示了蛋白质的金属结合位点中钙的结合。CaCl2对大肠杆菌微管蛋白样丝状温度敏感突变体Z(FtsZ)分裂蛋白聚合过程中总荧光的影响。coli进行了多孔荧光分析。

一般描述

Calcium chloride (CaCl2) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaClis used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaClis used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaClalong with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaClon the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.
 

应用

二水合氯化钙已用于:
  • 培养基制剂
  • 精子收集和制备
  • 细胞培养和原生质体制备

 

属性

产品线

BioReagent

质量水平

200

测定

≥99.0%

形式

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

溶解性

H2O: 294 mg/mL, clear, colorless

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

 

安全信息

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

商品规格
属性名称属性值
储存温度 Storage temp.常温阴凉避光
全球实时库存 Availability √美国St. Louis ≥ 99 | 欧洲Eur. ≥ 10 | 東京Tokyo ≥ 22 | 香港与北京 ≥ 32
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